RExPrimer | Tool name | RExPrimer |
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| URL | http://www4a.biotec.or.th/rexprimer/ |
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| Important features | 1. Designs Junction primers, sequencing primers, cloning primers [whole gene amplification primers can be designed without the restriction sites] and SNP primers.
2. Reports structural polymorphisms, which potentially cause PCR failure by checking target sequence with genomic variation databases. |
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| Citations | Piriyapongsa J, Ngamphiw C, Assawamakin A, Wangkumhang P, Suwannasri P,
Ruangrit U, Agavatpanitch G, Tongsima S. RExPrimer: an integrated primer
designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and
mis-priming from structural variation. BMC Genomics. 2009 Dec 3;10 Suppl 3:S4.
PubMed PMID: 19958502; PubMed Central PMCID: PMC2788391. |
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| Year of publication | 2009 |
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| Rank by usage frequency | 100 |
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| Comments | |
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| Function | Cloning primers, Junction primers, Sequencing primers, SNP primers, General PCR, Oligo analysis |
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| Category | Online, Free |
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| Features | 1. Shows the alignment of primers in relation to target
2. Allows for primer specificity checking and
3. Involves in SNP checking and avoids the regions with SNP's for primer designing.
2. Several databases like human SNP dbs, insertion deletion polymorphism dbs, pseudogene dbs and structural variation databases have been integrated in this. The primers generated are screened against these database. |
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| Description | |
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